操作步驟：

1. 標準(zhun)品(pin)的稀(xi)(xi)(xi)釋(shi)與加(jia)(jia)(jia)(jia)樣：在(zai)(zai)(zai)酶標包被板上(shang)設標準(zhun)品(pin)孔(kong)(kong)(kong)10孔(kong)(kong)(kong)，在(zai)(zai)(zai)di一(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標準(zhun)品(pin)100μl，然后(hou)在(zai)(zai)(zai)di一(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)(zhong)加(jia)(jia)(jia)(jia)標準(zhun)品(pin)稀(xi)(xi)(xi)釋(shi)液50μl，混勻(yun)；然后(hou)從di一(yi)孔(kong)(kong)(kong)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)取(qu)(qu)100μl分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)和第(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)，再在(zai)(zai)(zai)第(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標準(zhun)品(pin)稀(xi)(xi)(xi)釋(shi)液50μl，混勻(yun)；然后(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)和第(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)中(zhong)(zhong)先各(ge)(ge)取(qu)(qu)50μl棄(qi)掉，再各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)別(bie)(bie)(bie)取(qu)(qu)50μl分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)(zhong)，再在(zai)(zai)(zai)第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標準(zhun)品(pin)稀(xi)(xi)(xi)釋(shi)液50μl，混勻(yun)后(hou)從第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)(zhong)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)中(zhong)(zhong)，再在(zai)(zai)(zai)第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標準(zhun)品(pin)稀(xi)(xi)(xi)釋(shi)液50ul，混勻(yun)；混勻(yun)后(hou)從第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)分(fen)(fen)別(bie)(bie)(bie)取(qu)(qu)50μl加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)中(zhong)(zhong)，再在(zai)(zai)(zai)第(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標準(zhun)品(pin)稀(xi)(xi)(xi)釋(shi)液50μl，混勻(yun)后(hou)從第(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl棄(qi)掉。（稀(xi)(xi)(xi)釋(shi)后(hou)各(ge)(ge)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)樣量都(dou)為50μl，濃度分(fen)(fen)別(bie)(bie)(bie)為180 ng/L，120 ng/L ，60 ng/L，30 ng/，15 ng/L）。

2. 加樣：分(fen)別設空(kong)白孔（空(kong)白對照孔不加樣品(pin)(pin)及(ji)酶標(biao)試劑，其余各步操作相同）、待測樣品(pin)(pin)孔。在酶標(biao)包被板(ban)上待測樣品(pin)(pin)孔中先加樣品(pin)(pin)稀釋液40μl，然后再加待測樣品(pin)(pin)10μl（樣品(pin)(pin)最終稀釋度(du)為5倍）。加樣將樣品(pin)(pin)加于酶標(biao)板(ban)孔底部(bu)，盡量不觸及(ji)孔壁，輕(qing)輕(qing)晃動(dong)混(hun)勻。

3. 溫(wen)育：用封(feng)板膜封(feng)板后置37℃溫(wen)育30分(fen)鐘。

4. 配液(ye)：將(jiang)30（48T的20倍）倍濃縮洗(xi)滌(di)液(ye)用蒸餾(liu)水30（48T的20倍）倍稀釋后(hou)備用。

5. 洗滌：小心揭(jie)掉(diao)封(feng)板膜，棄去(qu)液(ye)體，甩干，每孔加(jia)滿洗滌液(ye)，靜(jing)置30秒(miao)后棄去(qu)，如此重(zhong)復(fu)5次，拍干。

6. 加酶：每孔加入酶標(biao)試劑(ji)50μl，空白孔除外。

7. 溫(wen)育：操作同(tong)3。

8. 洗滌：操作同5。

9. 顯(xian)(xian)色(se)(se)：每孔先加(jia)(jia)入顯(xian)(xian)色(se)(se)劑(ji)A50μl，再加(jia)(jia)入顯(xian)(xian)色(se)(se)劑(ji)B50μl，輕輕震蕩(dang)混勻，37℃避光顯(xian)(xian)色(se)(se)15分鐘.

10. 終止(zhi)(zhi)：每孔加終止(zhi)(zhi)液(ye)50μl，終止(zhi)(zhi)反應（此(ci)時藍色立轉(zhuan)黃色）。

11. 測定(ding)：以空白(bai)空調零，450nm波長依序測量各孔的吸光度(du)（OD值）。 測定(ding)應(ying)在加(jia)終止液后15分鐘。雌二醇單克隆抗體

雌三醇單克隆抗體

HRP標記雌三醇(chun)單(dan)克隆(long)抗體

甲基睪酮單克隆抗體

喹乙醇單克隆抗體

三聚氰胺單克隆抗體

賽庚啶多克隆抗體

金剛烷胺多克隆抗體

B1檢測試劑盒

M1檢測試劑盒

玉米赤霉烯酮(tong)檢(jian)測試劑盒

T-2毒素單克隆抗體

嘔吐毒素單克隆抗體

鹽酸克倫特羅檢測試劑盒

萊克多(duo)巴胺檢測(ce)試(shi)劑盒