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小鼠20SP elisa試劑盒操作步驟

小(xiao)鼠20SPelisa試劑盒操作(zuo)步驟(zou):1.標(biao)(biao)準品(pin)的稀釋(shi)與加(jia)樣：在(zai)酶(mei)標(biao)(biao)包被(bei)板上設標(biao)(biao)準品(pin)孔(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)，在(zai)di一(yi)、第二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)別(bie)加(jia)標(biao)(biao)準品(pin)100μl，然(ran)后在(zai)di一(yi)、第二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)加(jia)標(biao)(biao)準品(pin)稀釋(shi)液50μl，混(hun)勻(yun)；然(ran)后從di一(yi)孔(kong)(kong)(kong)(kong)、第二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)取100μl分(fen)(fen)別(bie)加(jia)到第三(san)孔(kong)(kong)(kong)(kong)和(he)第四孔(kong)(kong)(kong)(kong)，再在(zai)第三(san)、第四孔(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)加(jia)標(biao)(biao)準品(pin)稀釋(shi)液50μl，混(hun)勻(yun)；然(ran)后在(zai)第三(san)孔(kong)(kong)(kong)(kong)和(he)第四孔(kong)(kong)(kong)(kong)中(zhong)(zhong)先各(ge)取50μl棄掉，再各(ge)取50μl分(fen)(fen)別(bie)取50μl分(fen)(fen)別(bie)加(jia)到第七(qi)、第八(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)，再在(zai)第七(qi)、第八(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)別(bie)加(jia)標(biao)(biao)準品(pin)稀釋(shi)液50μl，混(hun)勻(yun)后從第七(qi)、第八(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)加(jia)到第五、第六孔(kong)(kong)(kong)(kong)中(zhong)(zhong)...

大鼠15脂加氧酶ELISA檢測試劑盒?操作步驟

大鼠15脂(zhi)加(jia)(jia)氧酶ELISA檢測(ce)試劑(ji)盒操作步(bu)驟:1.標(biao)準(zhun)品的(de)稀(xi)釋與加(jia)(jia)樣：在(zai)(zai)酶標(biao)包被板上(shang)設標(biao)準(zhun)品孔10孔，在(zai)(zai)di一(yi)、第(di)(di)(di)(di)(di)(di)二孔中(zhong)(zhong)(zhong)分(fen)別(bie)(bie)加(jia)(jia)標(biao)準(zhun)品100μl，然后(hou)在(zai)(zai)di一(yi)、第(di)(di)(di)(di)(di)(di)二孔中(zhong)(zhong)(zhong)加(jia)(jia)標(biao)準(zhun)品稀(xi)釋液50μl，混(hun)勻；然后(hou)從di一(yi)孔、第(di)(di)(di)(di)(di)(di)二孔中(zhong)(zhong)(zhong)各取(qu)(qu)100μl分(fen)別(bie)(bie)加(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)三孔和第(di)(di)(di)(di)(di)(di)四(si)孔，再(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)(di)四(si)孔分(fen)別(bie)(bie)加(jia)(jia)標(biao)準(zhun)品稀(xi)釋液50μl，混(hun)勻；然后(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)三孔和第(di)(di)(di)(di)(di)(di)四(si)孔中(zhong)(zhong)(zhong)先(xian)各取(qu)(qu)50μl棄掉，再(zai)各取(qu)(qu)50μl分(fen)別(bie)(bie)取(qu)(qu)50μl分(fen)別(bie)(bie)加(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔中(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔中(zhong)(zhong)(zhong)分(fen)別(bie)(bie)加(jia)(jia)標(biao)準(zhun)品稀(xi)釋液50μl，混(hun)勻后(hou)從第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔中(zhong)(zhong)(zhong)加(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)五、...

肺炎支原體pcr檢測試劑盒的核酸擴增原理

肺(fei)(fei)炎支原(yuan)(yuan)體是一(yi)(yi)種(zhong)常(chang)見(jian)的(de)呼吸道感染(ran)病(bing)原(yuan)(yuan)體，其(qi)感染(ran)可導致肺(fei)(fei)炎、支氣(qi)管炎等疾(ji)病(bing)。為(wei)了(le)準確、快速地(di)檢測出肺(fei)(fei)炎支原(yuan)(yuan)體感染(ran)，科(ke)學家們采(cai)用了(le)聚(ju)合(he)(he)酶(mei)鏈反應（PCR）技(ji)術(shu)。本文將(jiang)介(jie)紹肺(fei)(fei)炎支原(yuan)(yuan)體PCR檢測試劑盒(he)在核酸擴增方面的(de)應用。通常(chang)一(yi)(yi)個標準的(de)PCR檢測試劑盒(he)包括以下組分：1.DNA模板：從患者樣(yang)本中提(ti)取的(de)DNA。2.引物：特異于肺(fei)(fei)炎支原(yuan)(yuan)體基因序列(lie)的(de)短DNA段，用于引導DNA合(he)(he)成。3.dNTPs：四種(zhong)脫氧核苷酸，作(zuo)為(wei)DNA合(he)(he)成的(de)原(yuan)(yuan)料。4.聚(ju)合(he)(he)酶(mei)：一(yi)(yi)種(zhong)能催(cui)化DNA合(he)(he)成的(de)酶(mei)。5.PCR緩沖液：提(ti)供...

脫落酸（ABA）含量ELISA測試盒的存放以及注意事項

脫落酸(suan)（ABA）含量ELISA測(ce)試(shi)(shi)盒(he)的(de)存(cun)(cun)放以及注意事項(xiang):ELISA試(shi)(shi)劑(ji)盒(he)收到后立即儲存(cun)(cun)于(yu)2-8℃下：效(xiao)期見試(shi)(shi)劑(ji)盒(he)標(biao)簽；一切成分在2-8℃下可保存(cun)(cun)至(zhi)有(you)(you)效(xiao)期。運(yun)(yun)用(yong)前：一切試(shi)(shi)劑(ji)平衡至(zhi)室溫：不(bu)同批次的(de)試(shi)(shi)劑(ji)不(bu)能交流(liu)運(yun)(yun)用(yong)。1.停止液：1瓶(ping)：12ml：0.5M硫酸(suan)：儲存(cun)(cun)于(yu)2-8℃至(zhi)有(you)(you)效(xiao)期。2.胎球(qiu)蛋白(bai)A示蹤(zong)抗(kang)(kang)體(ti)：1瓶(ping)：0.6ml：濃縮：HRP符號的(de)抗(kang)(kang)人(ren)胎球(qiu)蛋白(bai)A示蹤(zong)抗(kang)(kang)體(ti)溶于(yu)穩定的(de)蛋白(bai)基(ji)質中(zhong)；此試(shi)(shi)劑(ji)運(yun)(yun)用(yong)前需用(yong)示蹤(zong)劑(ji)抗(kang)(kang)體(ti)稀(xi)釋液進行稀(xi)釋；儲存(cun)(cun)于(yu)2-8℃至(zhi)有(you)(you)效(xiao)期。3.包被板：96孔：包被了(le)抗(kang)(kang)人(ren)胎球(qiu)...

PCR試劑盒的回收率你理解多少？

PCR試劑盒是(shi)為PCR實驗(yan)設計(ji)的(de)(de)(de)預(yu)制混(hun)合物，包含了完成反(fan)應(ying)(ying)所需的(de)(de)(de)各種(zhong)成分(fen)。在討論PCR效(xiao)率(lv)時，一個(ge)重(zhong)(zhong)要的(de)(de)(de)概念(nian)就是(shi)“回(hui)收(shou)(shou)(shou)率(lv)”，這通常指的(de)(de)(de)是(shi)從(cong)樣(yang)本中(zhong)提(ti)取(qu)出的(de)(de)(de)DNA的(de)(de)(de)數量(liang)和(he)質量(liang)。理解(jie)回(hui)收(shou)(shou)(shou)率(lv)對于確(que)保實驗(yan)結果(guo)的(de)(de)(de)準確(que)性和(he)可重(zhong)(zhong)復性至(zhi)關重(zhong)(zhong)要。1.回(hui)收(shou)(shou)(shou)率(lv)的(de)(de)(de)含義回(hui)收(shou)(shou)(shou)率(lv)是(shi)指從(cong)起始樣(yang)本中(zhong)成功提(ti)取(qu)并(bing)用(yong)(yong)于PCR的(de)(de)(de)DNA的(de)(de)(de)比(bi)例。一個(ge)理想的(de)(de)(de)提(ti)取(qu)過程應(ying)(ying)該有很高的(de)(de)(de)回(hui)收(shou)(shou)(shou)率(lv)，這意(yi)味著樣(yang)本中(zhong)的(de)(de)(de)絕大部分(fen)DNA都被捕(bu)獲并(bing)可用(yong)(yong)于后(hou)續的(de)(de)(de)擴增(zeng)。2.影響回(hui)收(shou)(shou)(shou)率(lv)的(de)(de)(de)因素-樣(yang)本類型：不同(tong)類型的(de)(de)(de)樣(yang)本（如血液(ye)、組(zu)織、細胞、病(bing)毒顆粒等）具有...

大鼠 SCUBE1 ELISA檢測試劑盒操作步驟

大(da)鼠(shu)SCUBE1ELISA檢測試劑(ji)盒操作步驟：1.標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)的稀釋(shi)(shi)與(yu)加(jia)(jia)樣：在(zai)(zai)酶標(biao)(biao)包被(bei)板上設標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)孔(kong)(kong)(kong)10孔(kong)(kong)(kong)，在(zai)(zai)di一(yi)、第(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)100μl，然(ran)后在(zai)(zai)di一(yi)、第(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)加(jia)(jia)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)稀釋(shi)(shi)液50μl，混(hun)勻(yun)；然(ran)后從(cong)di一(yi)孔(kong)(kong)(kong)、第(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)各取100μl分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)到(dao)第(di)(di)(di)三(san)孔(kong)(kong)(kong)和第(di)(di)(di)四孔(kong)(kong)(kong)，再在(zai)(zai)第(di)(di)(di)三(san)、第(di)(di)(di)四孔(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)稀釋(shi)(shi)液50μl，混(hun)勻(yun)；然(ran)后在(zai)(zai)第(di)(di)(di)三(san)孔(kong)(kong)(kong)和第(di)(di)(di)四孔(kong)(kong)(kong)中(zhong)先(xian)各取50μl棄掉，再各取50μl分(fen)(fen)(fen)(fen)別(bie)(bie)取50μl分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)到(dao)第(di)(di)(di)七(qi)(qi)、第(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)，再在(zai)(zai)第(di)(di)(di)七(qi)(qi)、第(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)稀釋(shi)(shi)液50μl，混(hun)勻(yun)后從(cong)第(di)(di)(di)七(qi)(qi)、第(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)加(jia)(jia)到(dao)第(di)(di)(di)五、...

人原激活肽(TAP)酶聯免疫elisa試劑盒?洗滌方法

人原激(ji)活肽(TAP)酶聯免疫elisa試(shi)劑盒洗(xi)(xi)滌方法：1.自動洗(xi)(xi)板(ban)(ban)機(ji)：每孔(kong)(kong)(kong)(kong)加(jia)(jia)入(ru)洗(xi)(xi)滌液(ye)(ye)350μl，注入(ru)與(yu)吸(xi)出間(jian)隔60秒。洗(xi)(xi)板(ban)(ban)5次。2.手工洗(xi)(xi)板(ban)(ban)：甩盡孔(kong)(kong)(kong)(kong)內液(ye)(ye)體(ti)，在潔凈的(de)吸(xi)水(shui)紙上拍干，每孔(kong)(kong)(kong)(kong)加(jia)(jia)洗(xi)(xi)滌液(ye)(ye)350μl，浸泡(pao)1-2分(fen)鐘，吸(xi)去(不可觸及板(ban)(ban)壁(bi))或(huo)(huo)甩掉酶標板(ban)(ban)內的(de)液(ye)(ye)體(ti)，在厚的(de)吸(xi)水(shui)紙上拍干。洗(xi)(xi)板(ban)(ban)5次。實驗開始(shi)前(qian)，各試(shi)劑均應平衡至室(shi)溫(wen)；試(shi)劑或(huo)(huo)樣品(pin)配制時，均需充分(fen)混(hun)勻(yun)，并盡量(liang)避免起泡(pao)。1.加(jia)(jia)樣：分(fen)別(bie)設空白(bai)孔(kong)(kong)(kong)(kong)、標準孔(kong)(kong)(kong)(kong)、待測樣品(pin)孔(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)加(jia)(jia)樣品(pin)稀釋(shi)液(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)分(fen)別(bie)加(jia)(jia)標準品(pin)或(huo)(huo)待測樣品(pin)100μ...

Apelin 36ELISA試劑盒操作步驟

Apelin36ELISA試劑盒操作步驟:1.標(biao)準(zhun)(zhun)品(pin)的稀(xi)釋(shi)與(yu)加(jia)(jia)樣：在(zai)酶標(biao)包被板上設標(biao)準(zhun)(zhun)品(pin)孔(kong)(kong)10孔(kong)(kong)，在(zai)di一(yi)、第(di)(di)(di)二孔(kong)(kong)中(zhong)分(fen)(fen)別(bie)(bie)加(jia)(jia)標(biao)準(zhun)(zhun)品(pin)100μl，然(ran)后在(zai)di一(yi)、第(di)(di)(di)二孔(kong)(kong)中(zhong)加(jia)(jia)標(biao)準(zhun)(zhun)品(pin)稀(xi)釋(shi)液(ye)(ye)50μl，混(hun)勻(yun)；然(ran)后從di一(yi)孔(kong)(kong)、第(di)(di)(di)二孔(kong)(kong)中(zhong)各(ge)取100μl分(fen)(fen)別(bie)(bie)加(jia)(jia)到(dao)第(di)(di)(di)三(san)(san)孔(kong)(kong)和第(di)(di)(di)四孔(kong)(kong)，再在(zai)第(di)(di)(di)三(san)(san)、第(di)(di)(di)四孔(kong)(kong)分(fen)(fen)別(bie)(bie)加(jia)(jia)標(biao)準(zhun)(zhun)品(pin)稀(xi)釋(shi)液(ye)(ye)50μl，混(hun)勻(yun)；然(ran)后在(zai)第(di)(di)(di)三(san)(san)孔(kong)(kong)和第(di)(di)(di)四孔(kong)(kong)中(zhong)先各(ge)取50μl棄(qi)掉(diao)，再各(ge)取50μl分(fen)(fen)別(bie)(bie)取50μl分(fen)(fen)別(bie)(bie)加(jia)(jia)到(dao)第(di)(di)(di)七、第(di)(di)(di)八(ba)孔(kong)(kong)中(zhong)，再在(zai)第(di)(di)(di)七、第(di)(di)(di)八(ba)孔(kong)(kong)中(zhong)分(fen)(fen)別(bie)(bie)加(jia)(jia)標(biao)準(zhun)(zhun)品(pin)稀(xi)釋(shi)液(ye)(ye)50μl，混(hun)勻(yun)后從第(di)(di)(di)七、第(di)(di)(di)八(ba)孔(kong)(kong)中(zhong)加(jia)(jia)到(dao)第(di)(di)(di)五、第(di)(di)(di)六(liu)...